Journal: Nature communications

Article Title: Epithelial SIRT6 governs IL-17A pathogenicity and drives allergic airway inflammation and remodeling.

doi: 10.1038/s41467-023-44179-x

Figure Lengend Snippet: Fig. 7 | The SIRT6 inhibitor OSS_128167 (OSS) preventes airway remodeling in asthmatic mice. a Chemical Structure of OSS_128167 (OSS) is shown. b Schematic overview of experimental design for (b–j) in an ASA model. c Representative photomicrographs of lung inflammation expression are shown (Control n = 4; HDM + LPS n = 5, HDM + LPS + OSS n = 6). Scale bars, 100 μm. d, e The expression of RORγt, SIRT6, and IL-17A in the lung homogenate from mice treated with HDM/LPS or mice treated with HDM/LPS and SIRT6 inhibitor OSS_128167 (OSS) was analyzed by using Western blot. Quantification was analyzed by Image J software. f, g ELISA and qRT-PCR analysis of IL-17A in lung homogenate of mice. h–j The expression of inflammatory cytokines in the lung homogenate of mice was analyzed by using qRT-PCR or ELISA analysis. k Schematic overview of experimental design for (L-O)

Article Snippet: Human IL-17A, mouse IL-17A, CXCL1, CXCL2, IL-5, and IL-13 were measured using ELISA kit (Elabscience, China).

Techniques: Expressing, Control, Western Blot, Software, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Comparative performance of four LIT modes, Luminex, and ELISA

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Incubation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Schematic diagrams illustrating the methodologies used in Luminex® Assay ( a ) and ELISA ( b ). Figure was created with MedPeer (medpeer.cn). c Dose dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and Luminex (red). d Dose dependent median fluorescence intensity and absorbance of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and ELISA (red). Data presented as mean ± SD from three replicates. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Fluorescence, Standard Deviation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: a Dose-dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at various concentrations compared between Standard LIT assays with (red) and without (black) added serum. b Comparison of LODs for IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF with (pink) and without (blue) serum. c Spike recoveries for cytokines at high, medium, and low analyte concentrations. Linear regression plots comparing serum concentrations of IL-6 ( d ) and IL-8 ( e ) as determined by Luminex and LIT. Linear regression plots for IL-6 ( f ) and IL-8 ( g ) serum concentrations, determined by ELISA and LIT. Linear regression plots for IL-6 ( h ) and IL-8 ( i ) serum concentrations, determined by chemiluminescence and LIT. Data are presented as mean ± SD; n = 3 repeated tests. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Fluorescence, Comparison, Luminex, Enzyme-linked Immunosorbent Assay, Standard Deviation